Maulana Azad National Urdu University Tender

Supply and installation of LAB EQUIPMENT

SPECIFICATIONS FOR INVERTED FLUORESCENCE MICROSCOPE: a. The System should be Infinity‐corrected (RMS) threaded objectives with a 45 mm parfocal distance supporting fluorescence, brightfield, color brightfield, and phase contrast imaging modes, 60 mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli. b. The instrument should have five-position chamber for 4 fluorescence illuminators, LED light source with 50,000-hour life; broad choice of standard and specialty LED illuminators. (List of LED illuminators to be attached). c. Imaging methods by Single color, multicolor, time lapse, Z-stacking, movie capture, allowing to review, measure, and annotate captured images. and count cells in fluorescence mode post-acquisition. d. System should have mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel and lockdown holders to fix sample in place. List of available vessel holders (Well Plates, Dishes, Flasks and Slides) to be provided. e. The system must have automated focus mechanism with sub micron (0.150 um) resolution (single step accuracy)and mechanical focus wheel with single knob for coarse and fine focus. f. System must include 5 position objective turret with front mounted control and Plan Flourite 10x, 20x, objectives to be supplied along with instrument. g. The system must include 02 (GFP - 470/525 nm and RFP - 531/593 nm) independent high output LED illuminators to be supplied along with instrument have independent intensity control. h. The system must include high-sensitivity monochrome CMOS - 3.2 MP or better (2,048 x 1,536) camera with 3.45 μm pixel resolution. i. The system with small foot print with Dimensions (L x W x H) - 18 x 23 x 18 inches preferable and should have compatibility with the onstage Incubator j. System should include LCD Display - 18.5" articulated LCD color monitor with 1920x1080 pixel resolution with Embedded PC with 4 GB RAM. With 10GB SSD and 16GB USB 3.0 memory stick.

SPECIFICATION FOR FLUORIMETER: a. System should be compact fluorescence based quantification of DNA/RNA/ mircoRNA/ primers/NGS library and Protein and RNA Quality (RIN). b. System should have Large 5 -inch or better color touch screen for easy workflow navigation. c. System should have the sensitivity to measure picogram concentration of nucleic acids and utilize sample volume as low as 1ul. d. Stores up to 1,000 sample results on-board and more on given USB stick. e. Compatible plastic ware and standard needs to be provided for the calibration of the system. f. System should measure the intact RNA in less than 5 seconds per sample. g. System should have the below excitation and emission filters i. Excitation - Blue 430–495 nm, Red 600–645 nm ii. Emission - Green 510–580 nm, Red 665–720 nm. h. Vendor should support with compatible high sensitivity fluorescent dyes for quantification of DNA, RNA, Protein and RNA quality of samples. i. System should have a On-board pre programs and reagent calculator. j. System should have option to connect with WiFi for data transfer.