Bharathiar University Tender

Supply of Multimode Microplate Reader

Specifications for Multimode Microplate Reader General Wavelength Selection Should have Quadruple Monochromators. Should have Two excitation Monochromator and Two Emission Monochromator for wavelength selection. Detection method Should be capable to read Fluorescence, Time- resolved fluorescence, Luminescence and UV-Visible Absorbance. Read method Should be able to perform End-point, kinetic, spectral scanning, well-area scanning assays. Microplate types Should read 6, 12, 24, 48, 96 and 384-well plates without need for any adapters. Temperature control Ambient +4°C to 45°C + 0.2°C at 37°C. It should also have Condensation control system to minimize condensation on plate lids during incubation process. The incubation should be through natural heat convection to prevent edge effects in incubated assays. Shaking It should have linear, orbital, double orbital plate shaking modes. Also the speed and duration should be programmable. Software Single integrated windows based software for Reader control and data analysis should be supplied with the instrument. The software should be able to analyze the data and perform the calculations. Software must have Quick Read function to enable read the plate without lengthy protocol definition. Automation It should be upgradeable to interface with Microplate Stacker and also compatible with Automated CO2 / O2 Incubator Upgradable It should be upgradeable on site to include Anisotropy integrated in the same instrument for future needs Absorbance Light source Xenon Flash Lamp. Lamp Life should be at least 1 billion flashes Wavelength selection Monochromator Wavelength range 230 - 999 nm, 1 nm increment Bandpass 4 nm (230-285 m), 8 nm (285 m) Dynamic range 0 - 4.0 OD Resolution 0.0001 OD Pathlength correction Pathlength Correction facility should be available as a standard feature. Monochromator wavelength accuracy +/- 2 nm Monochromator wavelength repeatability +/- 0.2 nm OD accuracy 1% at 2.0 OD typical 3% at 3.0 OD typical OD linearity 1% from 0 to 3.0 OD typical OD repeatability 0.5% at 2.0 OD typical Stray light 0.03% at 230 nm typical Reading speed 96: 11 seconds 384: 22 seconds Fluorescence Intensity Sensitivity Top: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) Bottom: Fluorescein 4 pM typical (0.4 fmol/well 384-well plate) Light source Xenon Flash Lamp Wavelength selection Double grating monochromators (Top and Bottom) Wavelength range 250 – 900 nm Dynamic range 7 decades ( 0 – 9,999,999 RFU) Detection system Low noise PMT Gain Settings Should be able to program Auto or Manual gain settings for PMT Reading speed 96: 11 seconds; 384: 22 seconds Time Resolved Fluorescence Light Source Xenon flash lamp Wavelength range 250 – 900 nm Sensitivity Europium 1200 fM (120 amol/well in 384-well plate) Luminescence Sensitivity 20 amol ATP (flash) 100 amol ATP (glow) Wavelength range 300 - 700 nm Dynamic range 6 decades ( 0 – 5,800,000 RLU) Power Requirements Power 100-240 VAC 50/60 Hz . 130 Watts max Regulatory Regulatory Instrument should be CE and TUV marked.