All India Institute Of Medical Sciences Tender
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All India Institute Of Medical Sciences Tender
Healthcare and Medicine
GEM
Opening Date27 Mar 2024
Closing Date17 Apr 2024
Tender Amount₹ 10,34,243
Auction Start Date31 May 2024
Auction End Date1 Jun 2024
Costs
EMD
₹ 31,027Document Cost
Refer DocumentsTender Fee
Refer Documents
Description
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Contact
Tender Id
GEM/2024/B/4817622Bid Award Id
ViewTender No
GEM/2024/B/4817622Tender Authority
All India Institute Of Medical Sciences ViewPurchaser Address
-Website
http://bidplus.gem.gov.in
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Documents
molecular_biology_grade_chemicals_2024-03-26-12-04-20_aee3383e9d73faefe11affb377941399.pdf
boq_item_sample_file-39_2024-02-07-17-28-17_100374c_2024-03-26-12-04-20_01744df16f647606df9e642b9103bd76.csv
BOQ Items
TRIS BASE - MWt - 121.4 , 100 quality level,Molecular biology,7-9 PH range for DNA extraction 99.9 Percentage purity -Molecular Biology Grade
1
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TRIS HCl - MWt - 157.60 , Ultrapure,liquid,PH 7.5,1M For DNA extraction 99.9 Percentage purity -Molecular Biology Grade
1
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TRITON X 100 , Liquid form,detergent solution for DNA isolation 99.9 Percentage purity -Molecular Biology Grade
1
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AGAROSE , For routine separations of -100 bp to 20 kb,Genetic quality tested -GQT grade,Guaranteed to be free of DNA binders, inhibitors, DNases, and Rnases,very low gel background staining,very low sulfate content,very high gel strength and high exclusion limit and very low sulfate content 99.9 Percentage purity -Molecular Biology Grade
2
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RNAase A-175000 U , endonuclease free-100mg per ml, 7000 units per ml solution at 25 Degrees C and PH 5.0-99.9 Percentage purity -Molecular Biology Grade
3
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NUCLEASE FREE WATER , PH 6-8,Membrane filtered,Ultrapure -99.9 Percentage purity -Molecular Biology Grade
3
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BROMOPHENOL BLUE , Form-Powder,Pale pink to dark pink to red -99.9 Percentage purity -Molecular Biology Grade
3
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100 bp DNA LADDER- 1kb,ready to use, BP - 100 to 2000bp , 0.5micro grams per microliter,agarose gel type,100 applications,10 X Sample Loading Buffer -99.9 Percentage purity -Molecular Biology Grade
2
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GLYCOGEN , Liquid form,Molecular biology grade-99.9 Percentage purity -Molecular Biology Grade Pack Size-2x0.25 mL
2
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AMMONIUM ACETATE , 5M For RNA Extraction,liquid form -99.9 Percentage purity -Molecular Biology Grade
2
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PHENOL-CHLOROFORM-ISOAMYL ALCOHOL -25-24-1 , phenols,ultrapure,liquid form -99.9 Percentage purity -Molecular Biology Grade
2
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GLYCEROL , ultrapure,liquid form -99.9 Percentage purity -Molecular Biology Grade
1
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30 Percentage Acrylamide solution , 30 Percentage Acrylamide or Bis-acrylamide -37.5-1 -for Electrophoresis, Colorless clear liquid -99.9 Percentage purity -Molecular Biology Grade
2
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Ammonium Persulfate , For Protein Electrophoresis and Western Blotting -99.9 Percentage purity -Molecular Biology Grade
1
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TEMED solution , N, N,N,N-tetramethylethylenediamine for acrylamide gel preparation -99.9 Percentage purity Molecular Biology Grade
1
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Beta - mercaptoethanol , for Protein Labeling and Crosslinking,liquid form -99.9 Percentage purity -Molecular Biology Grade
1
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Glacial Acetic acid , 99.5-100.05 Percentage ,liquid form -99.9 Percentage purity -Molecular Biology Grade
1
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Protein ladder , 10 to 220 kDa size range,unstained. -99.9 Percentage purity -Molecular Biology Grade
2
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1X TMB , Elisa substrate,Horseradish peroxidase substrate type -99.9 Percentage purity -Molecular Biology Grade
2
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SYBR Green master mix , 2x Concentration,tube format,AmpliTaq Gold DNA Polymerase,premixed -99.9 Percentage purity -Molecular Biology Grade
1
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Sample loading Bromophenol Blue, Xylene Cyanol FF -XCFF for DNA loading buffer , 6X concentration,for Acrylamide Gels, Agarose Gels,5x1ml -99.9 Percentage purity -Molecular Biology Grade
2
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Sample loading bromophenol blue Dye for SDS PAGE , Denaturing Polyacrylamide Gels,liquid form,sample loading buffer -99.9 Percentage purity -Molecular Biology Grade
1
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Proteinase K - -graterthan 600 U Per mL -approximate 20 mg Per mL graterthan , 1ml,- -Graterthan600 U per mL -approximate 20 mg per mL graterthan ,liquid form -99.9 Percentage purity -Molecular Biology Grade
3
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Cell culture medium IMDM , Iscoves Modified Dulbeccos Medium-IMDM,Without L-Glutamine, HEPES, Sodium bicarbonate ,Off-white to Creamish white, homogenous powder -99.9 Percentage purity -Molecular Biology Grade
2
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Fetal Bovine serum , heat inactivated,sterile,filtered -99.9 Percentage purity -Molecular Biology Grade
1
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Antibiotic -Penicillin Per Streptomycin , 100x concentration,liquid form,12 months shelf life,sterile filtered,for prevention of cell culture contamination -99.9 Percentage purity -Molecular Biology Grade
2
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Tryphan blue , Blue,mammalian cells,0.004 concentration,liquid form,36 month shelf life,sterile-filtered,for cell counting -99.9 Percentage purity -Molecular Biology Grade
1
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MTT reagents-3--4,5-Dimethylthiazol-2-yl-2,5-Diphenyltetrazolium Bromide , for cell viability as a function of redox potential -99.9 Percentage purity -Molecular Biology Grade
2
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DMEM Medium , HeLa, 293, Cos-7, and PC-12 cell line-Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells,Sterile-filtered,With Additives,High Glucose,Glutamine Without Additives No HEPES, No Sodium Pyruvate For Use With -Application Mammalian Cell Culture, Glucose Concentration, 4500 mg per L -99.9 Percentage purity -Molecular Biology Grade
2
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RPMI -Roswell Park Memorial Institute 1640 Medium , HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas cell line,Leukemic Cells cell type,Animal origin free,1x concentration,Glutamine, Phenol Red
Without Additives,No HEPES, No Sodium Pyruvate,Sterile-filtered,Animal origin-free -99.9 Percentage purity -Molecular Biology Grade
2
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Colourless RPMI , HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas cell line,Leukemic Cells cell type,Animal origin free,1x concentration,Glutamine, NO Phenol Red Without Additives,No HEPES, No Sodium Pyruvate,Sterile-filtered,Animal origin-free -99.9 Percentage purity -Molecular Biology Grade
1
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Colourless IMDM , Iscoves Modified Dulbeccos Medium-IMDM,Without L-Glutamine, HEPES, Sodium bicarbonate and Phenol red,Off-white to Creamish white, homogenous powder -Molecular Biology Grade
1
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Colourless DMEM-Dulbeccos Modified Eagle Medium , HeLa, 293, Cos-7, and PC-12 cell line-Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells,Sterile-filtered,With Additives,High Glucose,Glutamine, Phenol Red, Without Additives, No HEPES, No Sodium Pyruvate, For Use With -Application, Mammalian Cell Culture, Glucose Concentration,4500 mg per L -Molecular Biology Grade
1
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Trypsin EDTA , with phenol red and EDTA additives ,Mammalian cells,Animal origin,1x concentration,270-320m0sm per kg,7.2-8 PH,Animal origin -Molecular Biology Grade
2
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Amphotericin , 0.25-2.5-micro gram Per mL,Liquid form,Anti-fungal,sterile-filtered,12 months shelf-life -Molecular Biology Grade
1
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HEPES Buffer , colorless,liquid form,PH-5.0-6.0-D20 or 4-1.071-1.075-N20 or D-1.370-1.372 -Molecular Biology Grade
2
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Disodium hydrogen phosphate , 98.0-100.5 Percentage purity, white -Molecular Biology Grade
2
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Sodium dihydrogen phosphate , 98.0-100.5 Percentage purity -Molecular Biology Grade
2
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Sucrose , ultrapure,for density gradient -99.9 Percentage purity -Molecular Biology Grade
2
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HhaI , Restriction Endonuclease enzyme,recognises GCG-C site and cuts at 10x Tango buffer at 37 Degrees C -99.9 Percentage purity -Molecular Biology Grade
3
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MspI , Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive,Restriction Endonuclease enzyme,recognises C-CGG site and cuts at 10x Tango buffer at 37 Degrees C -Molecular Biology Grade
3
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Taq DNA Polymerase including 10X Taq buffer-5U Per microliter , Amplification with minimal optimisation upt0 6 kb from genomic DNA and upto 20 Kb viral DNA includes 20 mM Mgcl2 with 10X dream Taq buffer 1.25 ml and 500 Units of Taq DNA Polymerase. -Molecular Biology Grade
2
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Diluent for DNA extraction-graterthan or equal to 99.80 Percentage Assay , For DNA elution,liquid form ,99.9 Percentage purity -Molecular Biology Grade
5
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RNA Later , stabilise and protect RNA with immediate RNAase inactivation -Molecular Biology Grade
2
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