Jawaharlal Institute Of Postgraduate Medical Education And Research Tender

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Jawaharlal Institute Of Postgraduate Medical Education And Research - JIPMER Tender

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Laboratory Equipment and Services
Chemical Products
Eprocure
Opening Date7 Oct 2024
Closing Date1 Nov 2024
Tender AmountRefer Documents 
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One step real time PCR mastermix for RNA viruses • One step Reverse Transcription real time PCR master mix (2x) compatible with TaqMan probes, *RT enzyme, *hot Start Taq DNA Polymerase *Nuclease free water and necessary sample buffers should be provided • Sample must be given for a minimum of 50 reactions

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cDNA synthesis kit • First strand cDNA synthesis kit containing Reverse Transcriptase • RNase Inhibitor • dNTP mix • Oligo dT primer • random hexamer primer, and buffer

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Hotstart conventional PCR mastermix • Conventional hot start PCR master mix • should be colored- ready to load directly from PCR tube to gel • Sample must be given for a minimum of 50 reactions

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PCR product gel purification kit (spin column) Spin column-based PCR gel purification kit with necessary buffers

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PCR product purification kit (spin column) Spin column-based direct PCR product purification kit with necessary buffers

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Molecular grade Agarose for gel electrophoresis 500g pack ·     Molecular grade

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Tris Borate EDTA buffer (10x) for gel electrophoresis · Application: Electrophoresis

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Tris EDTA buffer (1x), RNAse, DNase free molecular grade 100 ml · Molecular grade • DNase, RNase-free • ready to use • for reconstitution of primers

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Nucleic acid Extraction plasticware compatible for ILS GEN FAST (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb ) • Nucleic acid extraction • must provide compatibility certificate from OEM • U bottom • Sample must be given for a minimum of 3*96 reactions

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Probe 5'FAM + 3' BHQ-1™ HPLC, 10 nmol

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Probe 5'FAM + 3' BHQ-1™ 25nmol HPLC, 25 nmol

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Probe 5' HEX + 3' BHQ-1™ HPLC, 10 nmol

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Probe 5' HEX + 3' BHQ-25™ HPLC, 25 nmol

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Probe 5' TAMARA + 3' BHQ-2™ 10 nmol HPLC, 10 nmol

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Probe 5' TAMARA + 3' BHQ-2™ 25 nmol HPLC, 25 nmol

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Probe 5' ROX + 3' BHQ-2™ 10 nmol HPLC, 10 nmol

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Probe 5' ROX + 3' BHQ-2™ 25 nmol HPLC, 25 nmol

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Probe 5' Cy5® + 3' BHQ-2™ 10 nmol HPLC, 10 nmol

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Probe 5' Cy5® + 3' BHQ-2™ 25 nmol HPLC, 25 nmol

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MGB Probe 5' 6-FAM-labelled 6 nmol HPLC, 6 nmol

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MGB Probe 5' HEX-labelled 6 nmol

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DNA Base/ Per Base 10 nmol primer synthesis Conventional purification/HPSF

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DNA Base/ Per Base 25 nmol primer synthesis Conventional purification/HPSF

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3' Wobble (Degenerate bases) 40 nmol Conventional purification/HPSF

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Real time PCR plates • qPCR plates: Opaque • semi skirted, Low profile • 0.1ml volume • compatible with COBAS Z480 machine • should be provided with clear sealing film for real time PCR applications • should be DNase, RNase, and pyrogen free • must produce compatibility certificate from OEM • should provide 5 sample plates

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Real time PCR plates • qPCR plates: Ultra clear, * semi skirted, regular profile * 0.2ml volume, * compatible with QS5 and CFX96, *should be provided with clear sealing film for real time PCR applications * must produce compatibility certificate from OEM * should be DNase, RNase, and pyrogen free • Sample should be given for 5 plates

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Real time PCR strips • qPCR strips: opaque, low profile * 8well per strip, * must provide transparent strip caps * compatible with COBAS Z480 real time PCR* must produce certificate from OEM* should be DNase, RNase, and pyrogen free • Sample should be given for 5 strips

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Real time PCR strips • qPCR strips: Ultra clear, regular profile • 8well per strip • attached with transparent caps • compatible with QS5 and CFX96 real time PCR • must produce certificate from OEM • should be DNase, RNase, and pyrogen free • Sample should be given for 5 strips

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Sanger sequencing services Sequencing service for 1 sample includes PCR product purification, both forward and reverse strand sequencing

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100ul Filter tips Filter tips 100ul universal tips, Must be compatible with standard pipettes brands including Eppendorf, Thermofisher, Gilson and tarson.

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HEPES buffer 1 M cell culture grade, liquid.

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Sterile cell culture pipette (5 ml) Sterile, RNase-/DNase-free, Nonpyrogenic , Noncytotoxic , Exclusive anti-drip tip assures accurate delivery. Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier. Bidirectional graduations provide choice of ascending and descending scales. Negative graduations allow additional working volume. Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic Volumetric accuracy of ±2% at stated capacity

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Sterile cell culture pipette (10 ml) Sterile, RNase-/DNase-free, Nonpyrogenic , Noncytotoxic , Exclusive anti-drip tip assures accurate delivery. Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier. Bidirectional graduations provide choice of ascending and descending scales. Negative graduations allow additional working volume. Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic Volumetric accuracy of ±2% at stated capacity

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Cell culture medium EMEM Powdered media of Eagle's minimum essential medium (EMEM) L-Glutamine Without Sodium bicarbonate and Sodium pyruvate. Ready to reconsitute in 1 liter of distilled water.should provide 1 sachet as sample

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Cell culture medium DMEM Powdered media of Dulbecco's Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre and L-Glutamine Without Sodium bicarbonate and Sodium pyruvate. Ready to reconsitute in 1 liter of distilled water.should provide 1 sachet as sample

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Antibiotic, antimycotic solution (20 ml pack) Antibiotic Antimycotic Solution 100X liquid w/10,000 U Penicillin, 10mg Streptomycin and 25 µg Amphotericin B per ml in 0.9% normal saline Sterile filtered, sutable for cell culture. Exdotoxin tested.should provide 1 pack as sample

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Sterile Cell Scrapers • (With rotatable blade) Pivoting blades- 1.25cm Sterilized by gamma irradiation,Non-pyrogenic, non-toxic, DNase-Rnase.(individually wrapped) • should provide 5 scrapers as sample

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96-Well Flat-Bottom Plate 96-Well Flat-Bottom Plate, Tissue Culture- Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped) should provide 5 plates as sample

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6-Well Flat-Bottom Plate 6-Well Flat-Bottom Plate, Tissue Culture- Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped) should provide 5 plates as sample

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12 Well plate Flat-bottom plate. 12 well plate, flat bottom, polystyrene, sterile, with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped) should provide 5 plates as sample

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T-25 flask Flask: optically clear polystyrene Cap: non vented high-density polyethylene, Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 25 cm². Recommended working volume of 5-7 mL. Sterility by Gamma irradiation should provide 5 flask as sample

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T-75 flask Flask: optically clear polystyrene Cap: non vented high-density polyethylene, Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 75 cm². Recommended working volume of 15 - 22.5 mL. Sterility by Gamma irradiation should provide 5 flask as sample

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Trypsin-EDTA (0.25%), with phenol red (100 ml pack) Liquid formulation of trypsin contains EDTA and phenol red. Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination.

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Trypsin, TPCK Treated (25 ug/vial) Form-Powder, cell culture grade.

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V-bottom micro titre plate V-bottom clear wells, polystyrene, sterile, Nonpyrogenic , Noncytotoxic, with lid, packed individually. should provide 5 plate sample

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Conical Tubes (50 ml ) sterile Non- skirted, Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF. should provide 5 tube sample

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Conical Tubes (15 ml ) sterile Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF. should provide 5 tube sample

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Cell Freezing Medium-DMSO, 1X Without Antibiotics Sterile filtered, 50 ml pack For Cell preservation in liquid nitrogen

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Autoclavable transparent biohazard disposal bags- small size Clear transparent autoclavable bags Approximately 12*10 inches

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Kim wipes- lint free tissue Single ply light duty wiper *For cleaning equipments * Must be lint free Non abrasive

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Blotting sheet Purpose: General lab use Color: White/grey Size: 60*60cm Pack size: 100 sheets min

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ASPIRATOR BOTTLE WITH STOPCOCK capacity 5ltrs * autoclavable * medical grade PP *with stopcock

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ASPIRATOR BOTTLE WITH STOPCOCK capacity 10ltrs * autoclavable * medical grade PP *with stopcock

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CHEMICAL WASH BOTTLE Capacity- 500ml *Low density polyethylene material * corrosion resistant

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SELF-VENTING LABELED WASH BOTTLE- Ethanol Capacity- 500ml *Low density polyethylene material * corrosion resistant * have chemical name, international chemical society, chemical formula for " Ethanol" * non metallic *self venting * with color code

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SELF-VENTING LABELED WASH BOTTLE- Hypochlorite Capacity- 500ml *Low density polyethylene material * corrosion resistant * have chemical name, international chemical society, chemical formula for " Hypochlorite" * non metallic *self venting * with color code

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MEASURING BEAKER WITH HANDLE Capacity - 250ml * measurements written on the side *made of polypropylene material *autoclavable * sturdy to handel chemicals like phenol, acid etc *With embossed scale, blue graduations and spout

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MEASURING BEAKER WITH HANDLE Capacity - 500ml * measurements written on the side *made of polypropylene material *autoclavable * sturdy to handel chemicals like phenol, acid etc *With embossed scale, blue graduations and spout

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TEST TUBE BASKET WITH COVER basket model for use in autoclave, material polypropylene autoclavable, top opening - 180*170*160mm

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REAGENT RESERVOIR capacity 75ml, material polypropelene, autoclavable, wihtout divider

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RACK FOR MICRO TUBE made of polypropylene, capacity to hold 1.5ml microcentrifuge tube 20 places, autoclavable

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CRYO RACK - 50 PLACES self standing rack, for holding serum vials 1.8-2ml, 20-50 places, * Bottom of Each Self Standing Vials Get Interlocked In The Base Of The Rack. *Allows Single Handed Opening Or Closing Of The Vial Caps. *material: polycarbonate

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Cryo Tags 32.5 x 12.7 labels withstand conventional and cryogenic storage conditions.Freezer temperatures down to -196°C (liquid and vapor phase nitrogen), boiling water baths (100°C), autoclaving and moderate oven temperature (150°C). They will resist water soaking for at least 24 hours at room temperature and water vapor (100% relative humidity) at 35°C for at least 24 hours.

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sterile syringe filter filter with a 0.45 µm pore size, 33 mm, gamma sterilized individually packed PVDF material

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Fetal Bovine serum Research Grade, Sterile Filtered, Heat Inactivated ,Origin- US EU approved

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Digital probe thermometer Range : 4°c Refrigeration temperature range , Temperature measurement, sensor bases, with calibration option

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Digital probe thermometer Range : -20° C Freezer temperature range , Temperature measurement, sensor bases, with calibration option

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Digital probe thermometer Range : -70° C to -80 °C Ultra Freezer temperature range , Temperature measurement, sensor bases, with calibration option

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Card Board Cryo Box • Capacity - 25 places, holding place for 1.8ml cryo vials, withstand -80 temperature at ultra freezing storage condition • Suitable for air cargo shipment

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Cryo box • Capacity - 25 places, holding place for 1.8ml cryo vials, withstand -80 temperature at ultra freezing storage condition • Suitable for air cargo shipment

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Cryo cube box • Capacity - 50 places, holding place for 1.8ml cryo vials, withstand -80 temperature at ultra freezing storage condition, Autoclavable, made of polypropylene material • Suitable for air cargo shipment

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PCR Tube Rack Capacity - 96 places, holding place for PCR tube, withstand -80 temperature at ultra freezing storage condition, Autoclavable, made of polypropylene material

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Nitrile Gloves Small • Size: Small, Made of Nitrile, should be soft and comfortable to wear • should provide 5 pair as sample

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Nitrile Gloves medium • Size: Medium, Made of Nitrile, should be soft and comfortable to wear • should provide 5 pair as sample

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Nitrile Gloves large • Size: large, Made of Nitrile, should be soft and comfortable to wear • should provide 5 pair as sample

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Sample Bags • Size: 4" x 6" Made of Low-density polyethylene (LDPE), withstand -80 temperature at ultra freezing storage condition • Zip lock

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Sample Bags • Size: 5" x 8" Made of Low-density polyethylene (LDPE), withstand -80 temperature at ultra freezing storage condition • Zip Lock

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Serum sample cryogenic vials Capacity - 0.5 ml *made of polypropylene material, self stand Screw capped vial with silicon O ring, leak proof, sterile, DNase, RNase, Pyrogen free, Autoclavable

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Storage Vial Capacity - 5 ml *made of polypropylene material, self stand Screw capped, leak-proof, sterile, DNase, RNase, Pyrogen free, Autoclavable

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Specimen container Capacity - 125 ml *made of polypropylene material, self stand Screw capped, leak-proof, sterile, DNase, RNase, Pyrogen free

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Specimen container Capacity - 250 ml *made of polypropylene material, self stand Screw capped, leak-proof, sterile, DNase, RNase, Pyrogen free

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Iso-propanol • Density: 786 kg/m³ • Boiling point: 82.3 °C • Molar mass: 60.1 g/mol • IUPAC ID: propan-2-ol • molecular grade

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Tissue roll 2 ply Tissue rolls & 15 GSM Tissue roll: for normal laboratory use White in color

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Autoclavable transparent biohazard disposal bags- big size Clear transparent autoclavable bags Approximately 24*30inches

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11% hydrogen peroxide stabilized with silver suitable for fogging sterilization

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Laboratory glass thermometer Range : -10 to 50 °C Made of Glass with alcohol

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Mixed cellulose esters (MCE) • membrane plain filter, • 13 mm diameter, • hydrophilic, • white, • non-sterile • 0.22um • 3 sample must be provided

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Memberane filter Holder-47mm Material PSF, upper chamber 500ml, lower chamber 1000ml, Autoclavable

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