University Of Agricultural Sciences Tender

gkvk01 - Providing whole Genome Sequencing of 204 Plant Samples with following specifications: 1. Frozen leaf samples will be provided and DNA isolation from plant leaves should be performed by the vendor using the Qiagen plant isolation Kit only, 2. The total number of samples is 204, 3. Total DNA QC should be performed , 4. Whole genome sequencing ready library preparation should be performed, 5. Cluster amplification should be carried out, 6. Samples should be run on the NGS Sequencer DNB Seq T7 using DNA Nano ball technology to reduce duplication and sequencing should be performed on the Illumina navaseq 6000, 7. Sequence data of a minimum of 25Gb per sample should be provided, 8. Read length should be 150bp paired-end reads per sample (2x150bp format), 9. Upto 95% of the sequenced bases should have minimum Q30 value. Details specification and conditions enclosed separately.