Sher E Kashmir University Of Agricultural Sciences And Technology Tender
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Sher E Kashmir University Of Agricultural Sciences And Technology - SKUAST Tender
Agriculture or Forestry Works
Eprocure
Opening Date11 Feb 2025
Closing Date17 Feb 2025
Tender Amount₹ 38,00,000
Costs
EMD
₹ 76,000Document Cost
₹ 1,000Tender Fee
Refer Documents
Description
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Contact
Tender Id
2025_SKUST_272304_1Tender No
E-NIT-02-of-2025Tender Authority
Sher E Kashmir University Of Agricultural Sciences And Technology ViewPurchaser Address
-Website
http://http://www.skuastkashmir.ac.in/
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Documents
Procurement of Digital PCR (Droplet) under HADP Project- 21, at Division of Fruit Science, Main Campus Shalimar, SKUAST-K.
BOQ Items
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Digital PCR system should be equipped with a control software using a LCD Touch monitor
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System should able to use One plate with a multiplexing ability up to five channels, multiplexing up to 5 different dyes for single well without the need of customization of dyes or wavelength
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System should offer below Excitation and Emission wavelength for light source and detection system
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Digital PCR Should perform excitation and detection of up to 2 fluorescent dyes per partition (green, yellow).
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System should be equipped with an independent reference channel for counting the number of filled partitions and determining the analyzable volume.
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System should use high power white LED for illumination and image acquisition is performed using CMOS camera with 6.3 MP.
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Digital PCR system should analyze a full plate under 2 – 3 hours
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System should be compatible with probe-based detection chemistry and Eva green Chemistry, Vendor should provide validated Master Mixes to be used on Digital PCR System
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Digital PCR system Should work with Nano Plate technology, should be compatible with 8.5K and 26K nanoplates, which has a total of 8,500 and/or 26,000 partitions.
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Nanoplate should be in standard SBS format. The 8.5K nanoplate holds a total of 96 reactions (96-well) and the 26K nanoplate up to 24 reactions (24-well format) with minimum 8 wells.
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PCR well plate should have an input reaction volume: - 12 Microliter and 40 Microliter.
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Precision of ±10% should be achieved on the system
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Software should be accessed through a server network and also installed in individual computers for analysis purposes
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System software should allow users to access all images acquired by the digital PCR system for each channel
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Software of the system should offer data visualization as a table, histogram, scatterplot, heatmap and bar chart, Software should display fluorescence amplitude per partition in a 1D or 2D scatterplots (for multiplex data).
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System software should computes concentration in copies/ul for each sample.
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Software should automatically set thresholds across the entire plate or for individual wells. Alternatively, the user can manually set the thresholds.
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Software should offer data analysis for the calculation of mutation frequency, gene expression fold change, copy number variation as well as genome editing
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All analysis data and images in the software should exported as a .csv file (for use in Microsoft Excel or similar program) and .png or .svg (for images) and PDF Format
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Software of the system should be with guidelines of 21 CFR, vendor should provide document for the same as and when required
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Number of installations:- Vendor should have more than 20 installations in India.
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Warranty:- One year from date of the installation of the system
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Application training should be provided to two to three staff members by trained Application team of the Vendor / Manufacturer
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Note:-The rates quoted are inclusive of all carriages of materials (Mechanical/Manual) from source to site work and all extra charges complete in all respects.
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