Indian Institute Of Integrative Medicine Tender

STUDY-1

Study- I- Efficacy of Test compounds in rat model of bone cancer pain produced by breast cancer cells Animal Welfare: Studies to be done as per IAEC (CPCSEA) approved Protocols (1). Test System Species: Rat Strain: Wistar Sex, Age: Female, 6-8 weeks Procedure In anesthetized animals 1-cm-long skin incision to be made over the third trochanter. The rectus femoris muscle will be gently incised to expose the shaft of femur. A 23-gauge needle will be drilled vertically in the shaft of femur at the third trochanter level, allowing it to reach the intramedullary canal of the femur. 10 μl volume of Walker 256 cells (105 cells) or Hank's solution (sham group) will be injected. After slow injectiondrill hole will be sealed using bone wax. The muscle and skin will be stitched and disinfected. Treatment start: 3 days post surgery. Treatment end: 21 days. Efficacy Readout: • Body weights • mechanical allodynia • Behavioral tests • Observations: General clinical signs Analysis: One way ANOVA (95 % confidence level) Group Treatment No. of animals / group 1 Normal control 10 2 Disease Control 10 3 Reference Control 10 4 IIIM Reference Control-1*# 10 5 IIIM Reference Control-2*# 10 6 Test Dose -1# 10 7 Test Dose-2# 10 8 Test Dose -3# 10 *IIIM Reference Control 1 and 2 will be provided by CSIR-IIIM along with test compound

STUDY-2