University Of The Philippines Tender

Description Supply Of Liquid Chromatograph Tandem Triple Quadrupole Mass Spectrometer Ultra High-performance Liquid Chromatograph And Gas Chromatograph Single Quadrupole Mass Spectrometer (rebid) Terms Of Reference I. Lc-ms/ms; Triple Quadrupole Lc Tandem Ms -lc Must Have A Specification Published For Retention Time Repeatability Of <0.008 % Or <0.0008 Minutes; Area Repeatability < 1% Rsd -interface Must Provide Access To All Of The Following: • Connectivity To Check Status Or Run Diagnostics From Anywhere Within Your Network • Built-in Self-guided Diagnostics And Maintenance Capabilities • Method And Sequence Editing Without The Need For A Data System • Easy Access To Logs And Complete User Documentation • With Password Enable Feature To Limit Access To Authorized Lab Users Only • With Reliable Error And Warning System A. Two Triple Quadrupole Mass Spectrometer (ms1/ms2) Specification: Api Source And Optimization Mode: High Performances Heated Ion Sources: 1. Electron Ionization, Esi, Applicable Lc Flow Rate: 1 Ul/min To 2ml/min 2. Dual Ionization Source (esi And Apci), Dius Esi Desolvation Temperature: More Than 650˚c Apci Desolvation Temperature: More Than 500˚c Ion Optical System: Qarray Ion Guide And Two Quadrupoles For Efficient And Rapid Ion Transfer Or Equivalent Mass Analyzer: Two High Resolution Molybdenum Hyperbolic Mass Filters With Pre-rods Or Equivalent Collision Cell: Tapered Multipole Type Ultra-high Speed Collision Cell (uf Sweeper Iii Collision Cell) Or Equivalent Detector: Secondary Electron Multiplier With Off-axis Conversion Dynode Or Equivalent Vacuum System: Rotary Pump: 1-unit Triple Inlet Turbomolecular Pump Power Supply: Ms Unit: Ac 230 V 15 A (50/60 Hz) Single Phase Pc: Ac100 V (50/60 Hz) Hplc: Ac100v Performance Specification: Mass Range: M/z 2 To 2,000 Da Or Higher Maximum Scan Speed: 30,000 U/sec Or Higher (in All Modes Of Scanning) (0.1 U Step: 300,000 Data Points/sec) Maximum Dwell Time: 0.8 Sec Or Lower Minimum Pause Time: 1 Msec Or Lower Mrm Transition Speed: Maximum 555 Mrm/sec Mass Stability: The Mass Stability Is 0.05 Da Over 24 Hours Or Better Mass Accuracy: ±0.1 U Polarity Switching Time: 5 Msec Or Lower Cross Talk: ˂0.005% 1ug/ml Verapamil And Monitoring 455.2>165.0 And 470>165 Mass Resolution: R ˂0.7 U Fwhm And Adjustable To 0.5u Linear Dynamic Range: Typical 7 Order Or Better Mrm Sensitivity Esi [positive]: The Measure Signal-to-noise Ratio [rms] Obtained From The Chromatogram Monitoring The Transition M/z 609 To M/z 195 On Injection Of 1 Pg Of Reserpine Should Be More Than Or Equal To 850,000:1*, Based On A 1 Μl Injection Of A 1 Pg/μl Reserpine Solution In 70/30 Acetonitrile/water (h2o) (no Additive) At A Flow Rate Of 400 Μl/min In Mrm Mode. Mrm Sensitivity Esi [negative]: The Measured Signal-to-noise Ratio [rms] Obtained From The Chromatogram Monitoring The Transition M/z 321 To M/z 152.10 On Injection Of 1 Pg Of Chloramphenicol Should Be More Than Or Equal To 850,000:1 [rms], Based On A 1 Μl Injection Of A 1 Pg/μl Chloramphenicol Solution In Methanol (no Additive) At A Flow Rate Of 300 Μl/min In Mrm Mode. B. Ultra High-performance Liquid Chromatograph (front-end): Solvent Delivery Unit With Mixer Solvent Delivery Method: Parallel-type Double Plunger Plunger Capacity: 10 Μl Increments: 0.0001 Ml/min Gradient Type: High-pressure Flow Rate Accuracy: ≤± 1% (under Specified Conditions) Flow Rate Precision: ≤0.06% Rsd Or 0.02 Minsd, Whichever Greater Available Ph Range: 1 To 14 System Controller -features A Touch Panel And Allows The User To Control The Instrument And Carry Out Analysis Preparation Directly Without The Need For A Pc. -built-in Reservoir Tray -connectable Units: 8, (autosampler, Column Oven, Detectors, Etc.) On-line Degasser Number Of Degassed Solvents: Up To 3 Lines Degassed Flowline Capacity: 400 Ul/1 Line Degassing Performance: Using The Uv-absorption Detector, At Wavelength 210nm, Sensitivity 0.5au Or Better Operating Temperature Range: 4 To 35 ºc Column Oven - With Column Management Device Keeps Track Of Columns Easily. - Active Pre-heater Which Can Control The Temperature Of The Mobile Phase Before It Enters The Column, Improving The Separation Of Components With Short Retention Times. Cooling Method: Electronic Cooling Temperature Control Type: Forced Air Circulation Temperature Control Range: Room Temperature - 10°c To 85°c Temperature Control Accuracy: ±0.1°c Applicable Columns: 300 Mm X 3; 100 Mm X 6 Function: Change Of Temperature Setting Auto Sampler/ Auto Injector Maximum Operating Pressure: 130 Mpa Injection-volume Setting Range: 0.1 To 50 Μl (standard), 0.1 To 2,000 Μl (option) Number Of Processed Samples: 162 (1.5 Ml Vials) Injection Cycle Time: ≤6.7 Seconds (under Specified Conditions) Injection-volume Repeatability: Rsd ≤ 1.0% (0.5 To 0.9 Μl), Rsd ≤ 0.5% (1.0 To 1.9 Μl), Rsd ≤ 0.25% (2.0 To 4.9 Μl), Rsd ≤ 0.15% (more Than 5.0 Μl), Rsd < 0.5% (typically, 0.5 Μl), Rsd < 0.25% (typically, 1.0 Μl) Sample Carryover: ≤0.0015% (without Rinse); ≤0.0003% (with Rinse, Typically) Sample Cooler: Air-circulation Temperature Control Type, 4 To 40°c C. System Software Specifications - For Data Acquisition, Data Handling (quantitative/qualitative) -includes Automated Workflow For Setting Up And Starting Of Acquisition Of Samples Batches And Result Generation And Laboratory Information Management System (lims) Export -remote Access Processing Of Data Is Possible Ms Acquisition Modes: Q1 Scan (max 1000 Events), Q1 Sim (max 1000 Events X 32 Channel) Q3 Scan (max 1000 Events), Q3 Sim (max 1000 Events X 32 Channel) Msms Acquisition Modes: Product Ion Scans (max 1000 Events) Precursor Ion Scans (max 1000 Events) Neutral Loss Scans (max 1000 Events) Mrm (max 1000 Events X 32 Channels) Data Processing: Drawing Of Lc Chromatograms, Ms Chromatograms And Ms Spectra Elimination Of Background Noise In Lc Chromatograms Background Subtraction And Equalization For Ms Spectra Area Calculations For Lc Chromatograms And Ms Chromatograms Calculation Of Column Performance From Lc Chromatograms Instrument Control: Starting And Stopping The Ms Unit Automatic And Manual Adjustment Of The Ms Unit Lc Unit And Ms Unit Diagnosis Functions Data Management: Quantitative Analysis: -view And Compare Of Quantitative Chromatograms (ms Detector Data) -automatic Peak Detection Of Chromatograms -manual Peak Detection, Manual Identification, Delete Identification Results -quantitative Calculation Of The Identified Ions -draw Ms Spectrum For Identification -identification And Confirmation Using Library Spectra -display Calibration Curves, Edit Calibration Curves (add And Delete Calibration Points), Change Of Calibration Curve Type -display Quantitative Results, Filter Results -set Flagging Thresholds For Outliers Of Quantitative Calculation Results, Indicate Outliers With Colors And Flag Marks - Export Quantitative Results (tsv, Csv Format) -view User Specified Chromatograms -view User Specified Spectra -calculate Accurate Mass From Chemical Formula -function To Calculate A Chemical Formula From A Mass And Score Compared To The Real Spectrum -deconvolution Function To Obtain Zero Charge Spectra -search For Compounds In Ms Data Report Output: Quantitative Calculation Results, Quantitative Chromatogram, Sample Information, Calibration Curve, Ms Spectrum, Structural Formula Data Management: Qc Chart Display, Review Status Setting, Configure Precision For Quantitative Result Display; Perform Statistical Analysis – Identify Outliers Within A Sample Run, Or Spot Trends In Historical Data Across Many Sample Runs Libraries And Method Packages Included: • Lc/ms/ms Method Package For Primary Metabolites Such As Amino Acids, Acylcarnitines, Free Carnitine In Plasma, Urine, Cerebrospinal Fluid (csf) And/or Other Biological Materials -ready-to-use Analytical Conditions - With Two Selectable Analysis Methods - Protocols Are Included For The Preparation Of Extracts From Biological Tissue. Saving The User Time And Money, Even Laboratories Unfamiliar With Extraction Can Follow Prescribed Steps For Lc/ms/ms Sample Preparation -includes Optimized Analytical Conditions For Two Internal Standards To Permit Normalization Across Multiple Samples • Lc/ms/ms Method Package For D/l Amino Acids, Acylcarnitines • Nist Tandem Mass Spectral Library • Ms/ms Spectrai Fragmentation Libraries And Compound Databases For Clinical Research And Forensic Toxicology Sample • Database For Quantitative Lc/ms & Lc/ms/ms Method Information • Skyline Interface D. Computer Specifications: - Processor: Intel® Core I7-12800h - Memory: 32gb (2x16gb) Ddr4-3200 Necc Ram - Storage: 512 Gb Pcie Nvme M.2 Ssd; 1 Tb 7200 Rpm Sata Hdd - Hard Drivegraphics: Graphics: Nvidia Geforce Gtx 1660 Super (6 Gb Gddr6 Dedicated) Or Higher - 2 Units, 27” Or Larger, Flat, (oled Or Ips) Monitor (for Dual Screen System) - Windows 10 Os (64 Bit Pro) Or Higher - Wlan: Realtek Wi-fi 6 (2x2) And Bluetooth 5.2 Combo (supporting Gigabit Data Rate) Mu-mimo Supported - Bluetooth: Yes - With Ms Office H&s Lifetime Subscription - Wi-fi Wireless Dual Band Usb Adapter (fully Compatible With Windows 10) - Additional 1tb External Hard Drive - Ups, 1kva - Laserjet Colored Printer That Can Print With A Speed Of 712.10 Sec With A Maximum Resolution Of 600 X 600 Dpi For Black & Color; 800 Mhz Processor; Maximum Memory Capacity Of 128 Mb Ddr To Optimize The Performance; Has Hi-speed Usb 2.0 Port; Built-in Fast Ethernet 10/100base-tx Network Port & Wireless For Compatibility And One Year Of Warranty Facility. Other Inclusion: • Start-up Kit • 5 Packs Screw-thread Clear Vial With Write On Spot With Ptfe/white Silicone Septa (100pcs/pack) • Tq Standard For Auto Tuning • 5 Packs Syringe Filter, 0.22um • Gs Kit For Uplc O Precolumn Filter 0.2um, 1pk Or Equivalent O C18 1.8μm 2.1x50mm Or Equivalent O Vials For Lc/ms O Nonsterile Nylon Syringe Filters, Pore:0.22(μm), Housing Diameter:25(mm), 100/pk O Solvent Safety 4 Ports Bottle Caps Kit (2 Units) O Solvent Safety 2 Ports Bottle Caps Kit (3 Units) • 2 Pc Additional Lcms Column • 5 Packs Membrane Filter, 0.22 Um • Filtration Kit With Oil-less Vacuum Pump • Measurement Sample • Carrier Gas Supply Pipe • N2 Generator • Argon Gas (initial Supply) • Argon Gas Regulator • Vacuum Pump With (1) Free Pm Service • Parallel-redundant Uninterrupted Power Supply (1 Unit) Which Will Meet The Demand Of The Required Power Back Up Support For The Lcmsms Unit. • Hands-on Training Will Be Conducted By Factory Trained Engineers And Application Specialist To All Bgl Staff (local). • Includes All Expense Paid, Minimum Of 2 Weeks International Training (which Include Round Trip Airfare, Accommodations, Travel And Health Insurance And Cost Of Living Allowance, Meals, And Other Applicable Incidental Expenses) For Two (2) Bgl Staff At The Department Of Clinical Genetics At Erasmus Medical Center In Rotterdam, Netherlands (c/o Dr George Ruijter) Re: Training With Regards To The Uplc-ms/ms Method For The Quantification Of Glycosaminoglycans (gags), For The Diagnosis Of Mucopolysaccharidosis (mps). • Provides Analytical Methods For Analysis Of Metabolites In Biological Samples (blood, Urine, Intracellular Materials) And Acquisition Method With Fully Optimized Lc Parameters. • Warranty: 1 Year For Replacement Of Parts And 2 Years For Services With (2) Free Preventive Maintenance Service And (1) Free Calibration Service In Line With Iso Standards With Calibration Certificate • Service Support: Factory Trained Service Engineers With Minimum Of 5-year Experience • Should Entitle The Customer To Corresponding Discounts On Service/ Spare Parts Sale Compared To The Supplier’s Published Rates (after 2-year Service And 1 Year-on Parts, Warranty) • Certificate Of Calibration From The Manufacturer (in Accordance To Iso Guidelines/standards) E. Installation, Qualificaion And Calibration Of Lcms/ms Or Lc-ms Triple Quadrupole Lc-ms To The New Nih Building (10th Floor) With Gas Line • Gas Installation Package: Gas Line System Including The Tanks And Restraints And Gas Housing (safety Measure In Case Of Earthquake); Gas Shut Off Valve; And Gas Purifiers And Other Applicable Accessories • Installation Kit • Calibration Service With Certificate Of Calibration And Qualification (iso) • Warranty: 1 Year, Replacement Of Parts And Services, (2) Pm Service (semi-annual) Ii. Ultra High-performance Liquid Chromatograph With Photodiode Array (pda) And Spectrofluorometric Detectors - Equipped With An Automatic Pretreatment Function Including Sample Dilution And Reagent Addition. The System Can Be Set To Automatically Mix The Sample And Derivatization Reagents In The Autosampler Needle A. Ultra High-performance Liquid Chromatograph (front-end) Pda Detector • Wavelength Range Of 190 Nm - 800 Nm, Wavelength Accuracy ± 1 Nm, Noise Level Of 4.5 X 10-6 Au, And Drift Less Than 0.4 X 10-3 Au/hour. • Contains Both A Deuterium (d2) And Tungsten (w) Lamp. • Uv Cut-off Filter That Removes Wavelengths Below 240 Nm From Entering The Flow Cell. • Has A 1024 Element Diode Array. Fully Temperature-controlled Optics, Including The Optical Bench, Lamp Housing, And Flow Cell. Monitored And Controlled With A Standard Web Browser. • Built-in Cell Id That Is Automatically Recorded In Analysis Data. • Linear Up To 2.5 Au (astm Method) Or Better • Pressure Tolerance Of 12 Mpa Or Higher • Automatic Wavelength Accuracy Check And Wavelength Correction. • Integrated 4 Channel Analog Output. • Optional Flow Cells That Can Be Changed By The User: Uhplc, Semi-micro, Inert, Preparative And High-pressure Cell. • Capable Of Using The I-pdea Ii Deconvolution Algorithm To Resolve Co-eluting Peaks. Spectrofluorometric Detector • Wavelength Range Of 200 Nm - 650 Nm, Spectral Bandwidth Of 20nm, Wavelength Accuracy ± 2 Nm And Precision Of ± 0.2 Nm. • Contains Xenon (xe) Lamp. • Simultaneous Measurement Of Four Wavelengths, And Capable Of Wavelength Scanning. • Has Signal To Noise Ratio Of: Water Raman Peak S/n ≥ 200; Low Background S/n ≥ 9000. • With Standard Conventional Cell: Volume 12 Μl, Maximum Pressure 2 Mpa System Controller • System Controller Features A Touch Panel And Allows The User To Control The Instrument And Carry Out Analysis Preparation Directly Without The Need For A Pc. • Built-in Reservoir Tray • Connectable Units: 8, (autosampler, Column Oven, Detectors, Etc.) On-line Degasser • Has Flow Line Capacity Of 400 Μl Internal Volume Per Channel; Up To 5 Lines Of Degassed • Power Supplied From Pumps • Vacuum Level Can Be Displayed On Pump Control Panel Or Software • Maximum Operating Flowrate Of 10 Ml Per Minute Column Oven • Temperature Control Range Is (ambient - 10)°c To 85°c And The Temperature Setting Range Is 4°c To 85°c In 1°c Steps. • Temperature Accuracy Is ±0.8°c Or Better. • Temperature Control Precision Is ± 0.1°c Or Better. • The Column Oven Will Have Time Programming Capability In Stand-alone (320 Steps) Or System-controlled Configuration For Setting Temperature Ranges And Oven On / Off. • The Oven Can Store At Least 6 Columns Of 100 Mm Length Or 3 Columns Of 300 Mm Length. • The Oven Can Mount One Manual Injector, 2 Flow-channel Selection Valves (one Inside And One Outside), A Gradient Mixer And A Cell Block Of The Conductivity Detector. • The Oven Can Use A Mixer Recognition Device To Log The Size Of The Mixer Installed. • The Oven Can Communicate With An Optional Column Management Device To Track Column History. Solvent Delivery Module • Parallel Dual Micro Plunger (10 Μl) Construction For Low Pulsation And Stable Baseline. • Flow Rate Range From 0.1 Ul/min To 10 Ml/min With Accuracy Of ± 1% (whichever Is Larger), And Precision Within < 0.06% Or 0.02 Min Sd (whichever Is Larger) Of The Set Flow Rate. • Operable In Constant Flow Or Constant Pressure Mode, As Well As Isocratic, Binary High- Pressure Gradient, Ternary High-pressure Gradient, Or Quaternary Low-pressure Gradient Mode. • The Pump Can Display Total Operating Time, Volume Of Solvent Delivered For Each Seal, And Maintenance, Error And Operation Logs. • Maximum Pressure Of 130 Mpa/1300 Bar (3ml/min) With Ph Range Of 1-14. • With Unique Flowpilot Technology Or Equivalent That Ramps Up The Pump Flow Gradually, Waits For The Column To Reach Its Set Temperature, Then Ramps To The Set Flow Rate, Reducing Potential Column Damage From Pressure Shock. • With Flowpilot Automatic Diagnostic And Recovery Capabilities Or Equivalent To Allow Monitoring Of Pressure Fluctuations For Anomalies, Restoration Of Normal Operation, And Can Alert Users Of Low Solvent Levels. • Users Can Set Solvent Compressibility To Further Reduce Pulsation During Operation. • Internal Solvent Selection Valve (2 Or 4-channel) Or A Low-pressure Gradient Formation (lpge) Unit Can Be Added As Options. Mobile Phase Monitor • Reservoir Tray Weight Sensors Which Can Be Used To Monitor The Volume Of Mobile Phase Or Autosampler Rinse Solution In Up To Twelve Containers. The Containers Can Also Be Checked Remotely From A Smart Device. • With Notification From The Device If The Solvents Are Running Low • Consist Of The Following Unit: - Main Unit Controller - Main Unit Bottle Holder Autosampler • Features A Needle In The Flow Path Design To Minimize Carryover. • Standard Sample Loop (50 Μl), An Optional Sample Loop (2000 Μl) And A Fixed-loop Configuration (5 Or 20 Μl) Are Available. • Total Injection Method: 0.1 To 50 Μl In Increments Of 0.1 Μl From 0.1 To 9.9 Μl, And 1 Μl From 10-50 Μl. • Performs Sample Pretreatment Including Dilution, Reagent Addition, Co-injection Of Samples, Stacked Injection, And Overlapped Injection. • Temperature Control From 4 To 45°c (± 2°c Accuracy,) With A Forced Air Cooling Mechanism To Reduce Condensation. • Cycle Time Of ≤ 7 Seconds. • Injection Volume Repeatability: Rsd ≤1.0% For 0.5-0.9 Μl, Rsd ≤0.5% For 1.0-1.9 Μl, Rsd ≤0.25% For 2.0-4.9 Μl, Rsd ≤0.15% For Injections ≥5.0 Μl. • Injection Volume Accuracy Of ±1% For 5 Μl Injections (n=20). • Carryover Performance: 0.0015% (no Needle Rinsing) And 0.0003% (with Rinse) Or Lower. • Equipped With Both A Static Rinse Port And An Active Rinse Pump To Rinse The Outside Of The Needle Before And/or After Aspirating Sample. • A 3-solvent Internal Rinse Option Is Available To Clean The Inside Of The Needle And Loop After Sample Injection To Further Reduce Carryover. • A Dual Injection Mechanism Can Be Installed For High Throughput Operation Or Complementary Analysis. • Maximum Pressure Rating Of 130 Mpa (1300 Bar) Or Better. Software/data Management • Has A Tool Can Automatically Recognize Peaks Even In Complex Chromatograms With High Noise And Accounts For Baseline Drift For Higher Integration Accuracy • Has A Function That Can Quantify Peaks That Cannot Be Completely Separated By A Column. Separate Peaks By Simply Specifying The Time And Wavelength In The Software. • Has A Feature That Can Be Set To Start Up The Unit At A Specified Time, So That It Can Complete Auto-purge, Equilibration, And Baseline Checks In Advance. • Must Have The Capability To Set Up In Advance To Run Without User Intervention All The Way From Startup Through Analysis To Shut Down. • Can View The Status And Predict Analysis Completion Time For Multiple Systems From Any Location Thru A Smart Device Without A Need Of A Special Software. B. Computer Specifications: - Processor: Intel® Core I7-12800h - Memory: 32gb (2x16gb) Ddr4-3200 Necc Ram - Storage: 512 Gb Pcie Nvme M.2 Ssd; 1 Tb 7200 Rpm Sata Hdd - Hard Drivegraphics: Graphics: Nvidia Geforce Gtx 1660 Super (6 Gb Gddr6 Dedicated) Or Higher - 2 Units, 27” Or Larger, Flat, (oled Or Ips) Monitor (for Dual Screen System) - Windows 10 Os (64 Bit Pro) Or Higher - Wlan: Realtek Wi-fi 6 (2x2) And Bluetooth 5.2 Combo (supporting Gigabit Data Rate) Mu- Mimo Supported - Bluetooth: Yes - With Ms Office H&s Lifetime Subscription - Wi-fi Wireless Dual Band Usb Adapter (fully Compatible With Windows 10) - Additional 1tb External Hard Drive - Ups, 1kva - Laserjet Colored Printer That Can Print With A Speed Of 712.10 Sec With A Maximum Resolution Of 600 X 600 Dpi For Black & Color; 800 Mhz Processor; Maximum Memory Capacity Of 128 Mb Ddr To Optimize The Performance; Has Hi-speed Usb 2.0 Port; Built-in Fast Ethernet 10/100base-tx Network Port & Wireless For Compatibility And One Year Of Warranty Facility. Other Inclusions • Start-up Kit • 5 Packs Screw-thread Clear Vial With Write On Spot With Ptfe/white Silicone Septa (100pcs/pack) • 5 Packs Syringe Filter, 0.22um • Lc Kit For Uplc O Precolumn Filter 0.2um, 1pk O C18 1.8μm 2.1x50mm Or Equivalent For The Analysis Of Amino Acids O Second Column Suitable For Homocysteine, Xanthine, Hypoxanthine And Orotic Acid Analysis O Vial For Lc Analysis O Nonsterile Nylon Syringe Filters, Pore:0.22(μm), Housing Diameter:25(mm), 100/pk O Solvent Safety 4 Ports Bottle Caps Kit (2 Units) O Solvent Safety 2 Ports Bottle Caps Kit (3 Units) • 2 Pc Additional Uplc Column • 5 Packs Membrane Filter, 0.22 Um • 1 Unit Filtration Kit • 4 Pcs 1 L Mobile Phase Bottle • 1 Unit Dehumidifier 20l • Hands-on Training Will Be Conducted By Factory Trained Engineers And Application Specialist To All Bgl Staff (local). • Includes All Expense Paid, Minimum Of 2-week International Training (which Include Round Trip Airfare, Accommodations, Travel And Health Insurance And Cost Of Living Allowance, Meals, And Other Applicable Incidental Expenses) For Two (2) Bgl Staff At The Molecular Diseases Laboratory, Faculty Of Sciences, Autonomous University Of Madrid (c/o Dr. Pedro Salas) Re: 1) Interpretation And Quantification Of Organic Compounds In Urine; And 2) Quantification Of Methylmalonic Acid In Plasma, Galactitol And Galactonate In Urine, Very Long Chain Fatty Acids In Plasma, Acylcarnitines In Plasma, Lactate, Pyruvate, 3-oh-butyrate And Acetoacetate In Plasma And Of Galactose 1-phosphate Uridyltransferase In Erythrocytes. • Provides Analytical Methods For Analysis Of Metabolites In Biological Samples (blood, Urine, Intracellular Materials) And Acquisition Method With Fully Optimized Uplc Parameters. • Warranty: 1 Year For Replacement Of Parts And 2 Years For Services With (2) Free Preventive Maintenance Service And (1) Free Calibration Service In Line With Iso Standards With Calibration Certificate • Service Support: Factory Trained Service Engineers With Minimum Of 5-year Experience • Should Entitle The Customer To Corresponding Discounts On Service/ Spare Parts Sale Compared To The Supplier’s Published Rates (after 2-year Service And 1 Year-on Parts, Warranty) • Certificate Of Calibration From The Manufacturer (in Accordance To Iso Guidelines/standards) • 3 Kva Ups C. Reinstallation And Re-calibration Of Uplc To The New Nih Building With Gas Line • Installation Kit • Re-calibration Service With Certificate Of Calibration (iso) • Warranty: 1 Year, Replacement Of Parts And Services, (2) Pm Service (semi-annual) Iii. Gas Chromatograph Single Quadrupole Mass Spectrometer • Gc Must Have A Specification Published For Retention Time Repeatability Of <0.008 % Or <0.0008 Minutes; Area Repeatability < 1% Rsd • System Should Have A Technology That Uses Various Control Methods To Control Carrier Gas Flow To A Constant Flow Speed, Flow Rate, Or Pressure. • Four Detectors Can Be Installed Simultaneously And Individually Temperature Controlled. • Compatibility Of The Unit To Process Analytical Data Derived From The Laboratory’s Existing Unit Of Single Quadrupole Gc-ms • Interface Must Provide Access To All Of The Following: • Connectivity To Check Status Or Run Diagnostics From Anywhere Within Your Network • Built-in Self-guided Diagnostics And Maintenance Capabilities • Method And Sequence Editing Without The Need For A Data System • Easy Access To Logs And Complete User Documentation With Password Enable Feature To Limit Access To Authorized Lab Users Only With Reliable Error And Warning System • Pressure Has Typical Control Of ±0.001 Psi For The Range Of 0 To 150 Psi. Pressure Set Points May Be Adjusted In Increments Of 0.001 For The Range 0.000 To 99.999 Psi, And 0.01 For The Range 100.00 To 150.00 Psi With Integrated Leak Detector Function • With 7 Pressure Program Ramps • System Must Have A Split/splitless Inlet (s/sl), 0-150 Psi. Inlet Sealing System Is Built In Standard With Each S/sl Inlet For Quick, Easy, Injector Liner Changes In Under 30 Seconds. • System Is Capable Of Effluent Splitting, Back Flushing, And Column Switching Or Multiple Backflush Configurations. • The Programmed Rate Setting Range Should Be -250 To 250oc/ Min. Temperature Set Point Resolution: 0.1oc Or Better. The Oven Should Be Able To Cool Down In A Maximum Of 3.4 Min From 450oc To 50oc; With Highly Precise Oven Control • Possible To Program 32 Temperature Ramps, With Preset Oven Cooling Rates • Possible To Use Capillary Columns Of 50, 100, 250, 320 Microns And Above. • The Pressure Set Points Should Be Adjustable By Increments Of 0.001 Psi Up To 150 Psi. Maximum Temperature Attainable Should Be ≥450°c. • Split Ratio: Up To 9999.9:1 • Touchscreen User Interface • System Should Be Supplied With Computer That Has Minimum 16gb Ram, 480g Solid State Drive (ssd) And A Software, Which Is Based On Microsoft Windows 10 Operating System For Instrument Control, Data Acquisition, Data Analysis, Quantization, Automation & Customization With Online And Offline Sessions Provided. • System Should Be Designed And Manufactured Under Iso9001 And Should Comply With Most Of International Regulatory, Safety And Electromagnetic Compatibility Requirement A. Specifications: Ion Source • Ionization: Ei (standard) • Temperature: 140 To 350 °c • Filament: Dual, Automatic Switching • Electron Energy: 10 To 200 Ev Or Better • Emission Current: 5 To 250 Μa Or Better Vacuum System • Main Pump: Turbo Molecular Pump Differential Exhaust System • Auxillary Pump: Oil Rotary Pump, 30 L/minute (60 Hz) • Column Flow: 15 Ml/minute (he) Mass Analyzer And Detector • Mass Analyzer: Metal Quadrupole Mass Filter With Pre-rods • Mass Range: M/z 1.5 To 1090 Or Higher • Mass Resolution: 0.4 To 2.0 U (fwhm) • Mass Axis Stability: ±0.1 U/48 Hours (constant Temperature) • High-speed Scan Control: Advanced Scanning Speed Protocol • Scan Rate: 20,000 U/second Or Better • Minimum Measurement Interval: 0.01 Sec (100 Scans/second Maximum) • Detector: Electron Multiplier With Low Noise Auto Sampler • Must Be Have A Minimum Of 16 Vials Auto Sampler Or Better, For Washing And Other Reagents For Standard Addition; Must Be Able To Accommodate A Minimum Of 150 Samples Per Batch Run • Injection Volume Range: 10nl To 200 Ul • Injection Linearity: >0.999 Coefficient Of Deviation • Maximum Run Time: 9999.99 Min. • Sample Delivery Precision: ≤1.00% Rsd • Sample Carryover: <0.005% Or 5ppm • Safety Mechanics: Leak Sensor (if Applicable) And Self-diagnosis Function Column And Column Oven • Column: 5sil Ms, 30m, 0.25mm, 0.25um Or Equivalent Depending On Manufacturer’s Brand • Column Temperature Control: Up To 450°c • Column Tracking: Column History/ Information Tracking System Software Specifications • With Software Platform To Support Data Generation And Processing: • Availability Of Quantification Methods Database; Up To 10 Libraries Configuration; Installation Of 3 Spectral Libraries • Should Have Capability Of Locking/ Adjusting The Retention Time So That Same Retention Time Can Be Reproduced From System To System And The Method Should Be Electronically Transferred. • Includes Automated Workflow For Setting Up And Starting Of Acquisition Of Samples Batches And Result Generation And Laboratory Information Management System (lims) Export • Remote Access Processing Of Data Is Possible • Data Management: Qc Chart Display, Review Status Setting, Configure Precision For Quantitative Result Display; Perform Statistical Analysis – Identify Outliers Within A Sample Run, Or Spot Trends In Historical Data Across Many Sample Runs B. Computer Specifications: - Processor: Intel® Core I7-12800h - Memory: 32gb (2x16gb) Ddr4-3200 Necc Ram - Storage: 512 Gb Pcie Nvme M.2 Ssd; 1 Tb 7200 Rpm Sata Hdd - Hard Drivegraphics: Graphics: Nvidia Geforce Gtx 1660 Super (6 Gb Gddr6 Dedicated) Or Higher - 2 Units, 27” Or Larger, Flat, (oled Or Ips) Monitor (for Dual Screen System) - Windows 10 Os (64 Bit Pro) Or Higher - Wlan: Realtek Wi-fi 6 (2x2) And Bluetooth 5.2 Combo (supporting Gigabit Data Rate) Mu- Mimo Supported - Bluetooth: Yes - With Ms Office H&s Lifetime Subscription - Wi-fi Wireless Dual Band Usb Adapter (fully Compatible With Windows 10) - Additional 1tb External Hard Drive - Ups, 1kva - Laserjet Colored Printer That Can Print With A Speed Of 712.10 Sec With A Maximum Resolution Of 600 X 600 Dpi For Black & Color; 800 Mhz Processor; Maximum Memory Capacity Of 128 Mb Ddr To Optimize The Performance; Has Hi-speed Usb 2.0 Port; Built-in Fast Ethernet 10/100base-tx Network Port & Wireless For Compatibility And One Year Of Warranty Facility. Other Inclusion: • Start-up Test Kit/s, 300 Pcs. Non Pre-slit Septa 2ml Glass Vial • Nist Ms Library (latest Version), Wiley, Fiehn Gc/ms Metabolomics Rtl Library And Kit, Smart Metabolite Database Or Equivalent; Relevant To Pharmaceutical And Biochemical Compounds And Comprehensive Database For Clinical Biomarkers; Include Tandem (ms/ms) Libraries And Gc Methods/retention Indices Library With Provision Of Free Updating Of The Library Up To Three Years From The Date Of Delivery. • Install Kit For Gcs With Gas Purifiers; Gas Filter And Regulator For Gases • Vial, Screw, 2ml, Amber, 100/pk And Blue Screw Caps, 100/pk And Column Based On Application • Spare Supplies/accessories Which Include But Not Limited To: 1) Syringe 10 Ul For Liquid Injection, 2) Supply Of Vacuum Oil; 3) O-rings; 4) (1) Additional Analytical Column Of Same Specification; 5) Vials For Washing And Waste Solvent And 6) One (1) Additional Column For Other Clinical Diagnosis Of Iem Applications Of The Gc-ms; 7) (1) Set Of Gas Purifiers; 8) Pm Kit; 9) One Unit 1 Tb External Hard Drive For Storage Of Analytical Data (back Up); 10) Table For The Unit; 11) Equipment Standards And Tuning Solutions • Parallel-redundant Uninterrupted Power Supply (1 Unit) Which Will Meet The Demand Of The Required Power Back Up Support For The Gcms Unit. • Vacuum Pump With (1) Free Pm Service • Hands-on Training Will Be Conducted By Factory Trained Engineers And Application Specialist To All Bgl Staff (local At Least 3-5 Days With Hands On). • Includes All Expense Paid, Minimum Of 2 Weeks International Training (which Include Round Trip Airfare, Accommodations, Travel And Health Insurance And Cost Of Living Allowance, Meals, And Other Applicable Incidental Expenses) For Two (2) Bgl Staff Training At Sheffield Childrens Hospital Inherited Metabolic Disease Laboratory In The United Kingdom (c/o Drs Jim Bonham, Simon Olpin And Nigel Manning) Re: 1) Synthesize 1,514c Glutaryl-coa From 1,514c Glutaric Acid And Set Up And Run The Assay On Cultured Fibroblasts Via Trapping Released 14co2 In Fiber-glass Filter Discs And Quantifying The Captured Radioactivity By Beta Emission ; 2) Overview Of Software Applications To Facilitate Processing And Interpretation Of Urine Organic Acid Analysis; 3) Two Dimensional Tlc For Amino Acid Screening In Body Fluids; 4) Fibroblast Immunofluorohistochemical Staining For Catalase In The Screening For Peroxisomal Disorders; 5) Fibroblast Fillipin Staining For Niemann Pick Type C Disease; 6) Very Long Chain Fatty Acid Analysis By Gcms, Methodology And Aspects Of Method Development And 7) Demonstration Of Interpretation And Validation Of Acylcarnitine Profiles. • Provides Analytical Methods For Analysis Of Metabolites In Biological Samples (blood, Urine, Intracellular Materials) And Acquisition Method With Fully Optimized Gc Parameters. • Warranty: 1 Year For Replacement Of Parts And 2 Years For Services With (2) Free Preventive Maintenance Service And (1) Free Calibration Service In Line With Iso Standards With Calibration Certificate • Service Support: Factory Trained Service Engineers With Minimum Of 5-year Experience • Should Entitle The Customer To Corresponding Discounts On Service/ Spare Parts Sale Compared To The Supplier’s Published Rates (after 2-year Service And 1 Year-on Parts, Warranty) • Certificate Of Calibration From The Manufacturer (in Accordance To Iso Guidelines/standards) C. Installation, Qualification And Calibration Of Gcms To The New Nih Building With Gas Line • Gas Installation Package: Gas Line System For Nitrogen, Helium And Argon Including The Tanks And Restraints And Gas Housing (safety Measure In Case Of Earthquake); Gas Shut Off Valve; And Gas Purifiers And Other Applicable Accessories • Installation Kit With Gas Purifiers • Calibration Service With Certificate Of Calibration And Qualification (iso) • Warranty: 1 Year, Replacement Of Parts And Services, (2) Pm Service (semi-annual) Other Inclusions (for All 3 Equipment):  Suppliers To Provide Certification Re: Exemption From Ivd Registration For All The Above Equipment.  Supplier Must Be Able To Provide Evidence/s On The Actual Use Of The Equipment Model Aligned With The Analysis Of Human Samples, Such As Plasma, Urine, Cerebrospinal Fluid And Others, For The Diagnosis Of Inborn Errors Of Metabolism.  Suppliers To Provide Opportunity For Site Visit In Laboratories In The Philippines With Installation Of Each Equipment Being Offered  Suppliers To Provide Details Pertinent To The Following: O Cost Of Pm Per Year To Include Options For 1 Lot For The 3 Equipment O Cost Of Software Upgrade After The Warranty Period  Each Software Must Be Subject To Maintenance Plan For At Least 1 Year.  Supplier To Provide Free Software Upgrade For At Least 2 Years From The Date Of Installation/first Use.  Supplier To Interface Software With Ihg’s Existing Laboratory Information Management System (lims) For Automated Test Report Preparation  Delivery Period: Within 120-180 Calendar Days From The Date Of Receipt Of P.o.  Price Is Vat Inclusive. Note: Suppliers/bidders Should Participate In Blind Testing Where Biochemical Genetics Laboratory Is To Provide 2 Samples Per Instrument That They Are Going To Test The Following Using The Same Equipment And Methodology That Will Be Included In The Bid And Submit The Results Together With Their Bid Documents. The Purpose Of This Is For The Laboratory To Be Able To Assess The 1) Demonstrated Sensitivity And Precision Of The Equipment; 2) Demonstrated Capability To Detect Biomarkers Involved In The Screening And Diagnosis Of Patients With Inherited Metabolic Disorders; 3) Demonstrated Capability Of The Supplier To Provide Technical Support To Biochemical Genetics Laboratory. Instrument Application Data Output Lc-ms/ms; Triple Quadrupole Lc Tandem Ms Plasma Acylcarnitine Analysis Quantitative Ultra High Performance Liquid Chromatograph Plasma Amino Acid Analysis Quantitative Gas Chromatograph Single Quadrupole Mass Spectrometer Urine Organic Acid Analysis Semi-quantitative Results Of Analysis Must Indicate The Following: 1. Instrument Used - Instrument Detection Limit 2. Analytical Method Used – Method Detection Limit 3. Analytes Detected – To Include The Chromatograms And Raw Data Generated By The Equipment 4. Concentrations/levels Of Analytes Detected Per Measurement (at Least 2 Trials)