University Of Ottawa Tender

The Unversity Of Ottawa Is Pleased To Invite Qualified Proponents To Submit A Proposal For The Provision Of One (1) Automated Digital Polymerase Chain Reaction (dpcr) With All The Necessary Proponents And Software For Absolute Quantification Of Gene Expression. The University’s Research Program Will Use This Equipment For Absolute Quantification Of Gene Expression Across A Range Of Biological Samples. An Automated Digital Pcr System Facilitates The High-throughput Precision Detection And Quantification Of Specific Nucleic Acid Sequences Within A Sample. A Dpcr Process Is Based On Combining Dna Samples With Primers, Probe Dyes And Pcr Reagents. Nucleic Acid Samples Are Separated Into Thousands Fixed Volume Partitions, Each Of Which Serves As An Independent Pcr Reactions. Target Sequences Undergo Amplification Following A Standard Pcr Principles And Are Identified Via Fluorescence Detection Of Non-specific Flourophores Or Sequences Specific Probes.  A Dpcr System Is A Workhorse Equipment In Many Molecular Biology Labs That Provides Absolute Quantification Of Of Target Dna Sequences Within A Sample For Applications That Include Mutation Detection, Gene Expression Analysis, Genome Edit Detection, Copy Number Variation Studies, Multiplex Mutation Screening, Viral Titer Measurement, Contamination Testing, And Research Into Infectious Diseases.  We Request An Automated Digital Prc System To Be Housed In The Core Molecular Biology And Genomics Laboratory (cmbgl) At The University Of Ottawa. This Equipment Will Accommodate The Experimental Needs Of More Than 36 Research Groups Across Several Faculties At The University (>100 Users).  We Require A High Level Of Automation And Ease Of Usage To Increase The Capacity Of The Facility For Various Experiments. Finally, This Equipment Will Be Managed By A Trained Technician That Will Ensure Accessibility To Hqp From Various Labs, Departments And Faculties Across Uottawa, As Well As Users External To The University.