Kerala State Council For Science Technology And Environment Tender

Low Depth Whole Genome Sequencing

Project execution strategies Samples 9 individual leaf samples of Teak Instrument Illumina or PacBio ONSO plaform Application Low-depth genome sequencing of nine Teak samples and SNP variant calling. Sample quality control Qubit and Agarose Library preparation Illumina/Pacbio library preparation Kit Number of Libraries 9 Nos. Library validation and QC BA/Qubit Read length 2X150 bp (PE sequencing) Sequencing mode High output Data generation per sample 6 Gb per sample with Q3085 to 90 % per sample Depth of sequencing per sample 20X per sample SNP variant calling GATK4 Pipeline Deliverables 1. DNA QC report 2. Library preparation report 3. Fast QC report and Sequencing output 4. Raw data (FastQ and Fasta format), is to be provided through FTP protocol, having read me file with the explanation. 5. SNP variant calling (Raw VCF, VCF for call and final VCF) and CSV report 6. A detailed report is to be submitted after completion of the experiment.